|
KMID : 0368420050480040404
|
|
Journal of Plant Biology
2005 Volume.48 No. 4 p.404 ~ p.410
|
|
|
Effects on GSH synthesis in Chinese cabbage when the culturing solution is supplemented with ammonium sulfate or the constituent amino acids for glutathione
|
|
Kim Ju-Sung
Seo Sang-Gyu
Kim Sun-Hyung
Usui Kenji
Shim Ie-Sung
|
|
|
Abstract
|
|
|
|
We examined the effects of the constituent amino acids for glutathione (GSH) ? glutamate (Glu), cysteine (Cys), and glycine (Gly) ? on GSH synthesis in Chinese cabbage seedlings. Glu, Cys, and Gly were applied simultaneously (100 mg L-1) to the culture solution for 2 d. When compared with the control, GSH concentrations were increased by 2.1-fold (640.4 nmol g-1 FW) and 1.5-fold (416.4 nmol g-1 FW) in the first leaf and the roots, respectively. Of all the free amino acids, the non-essentials, including Glu, Cys and Gly, occupied 95.5% (shoots) and 81.9% (roots) of the total. Cys supplements greatly enhanced the GSH concentration in the roots; application of 100 mg L-1 increased the level by 7-fold over the control. The activity of GSH synthetase was higher in the roots than in the leaf, whereas that of y-glutamylcysteine synthetase was higher in the leaf.
|
|
|
KEYWORD
|
|
|
Chinese cabbage, cysteine, glutathione, glutathione synthetase, y-glutamylcysteine synthetase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|